Hotspot

rps16-trnQ-UUG

LSC · 5505-7736 · 2232 bp

Back to Helwingia

Pi 0.000597
MarkerSeek score 57.70
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
2232
variable_sites
2
pi
0.000597
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 TTCCTTCTCTTCGGGATCGA GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 2307-2308 bp 1.0 78.4
rps16-trnQ-UUG_p2 GCAACGATTTGATAGACGGC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.6 58.1 2278-2279 bp 1.0 76.6
rps16-trnQ-UUG_p3 CGGGATCGAACATCAATTGC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.6 58.1 2296-2297 bp 1.0 76.4
rps16-trnQ-UUG_p4 CTTCCTTCTCTTCGGGATCG GAGGTTCGAATCCTTCCGTC 55.0 55.0 57.5 58.1 2308-2309 bp 1.0 75.5
rps16-trnQ-UUG_p5 TTCCTTCTCTTCGGGATCGA TTCGGAGGTTCGAATCCTTC 50.0 50.0 57.9 57.3 2311-2312 bp 1.0 73.7