primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	CCGTGCTAACCTTGGTATGG	20	20	55.0	55.0	58.1	58.3	0.0	0.0	0.465225	80	402	441-447	1.000000	77.5
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.469587	80	402	455-461	1.000000	77.5
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.471094	80	402	454-460	1.000000	77.5
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.471094	80	402	456-462	1.000000	77.4
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	GATCCACTTGGCTACATCCG	ACCGTGCTAACCTTGGTATG	20	20	55.0	50.0	58.1	57.6	0.0	0.0	0.525510	80	402	442-448	1.000000	77.1
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	TTCCTTCTCTTCGGGATCGA	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.9	58.1	0.0	16.9	0.215424	5505	7736	2307-2308	1.000000	78.4
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	GCAACGATTTGATAGACGGC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.6	58.1	0.0	16.9	0.445081	5505	7736	2278-2279	1.000000	76.6
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	CGGGATCGAACATCAATTGC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.6	58.1	0.0	16.9	0.465814	5505	7736	2296-2297	1.000000	76.4
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	CTTCCTTCTCTTCGGGATCG	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	57.5	58.1	0.0	16.9	0.576610	5505	7736	2308-2309	1.000000	75.5
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	TTCCTTCTCTTCGGGATCGA	TTCGGAGGTTCGAATCCTTC	20	20	50.0	50.0	57.9	57.3	0.0	16.9	0.806797	5505	7736	2311-2312	1.000000	73.7
psbK-psbI_p1	psbK-psbI	1	GTTTGGCAAGCTGCTGTAAG	CGTCCTGGATCATTAGAGAGG	20	21	50.0	52.4	57.9	57.1	1.4	0.0	1.988639	8325	8719	514-516	1.000000	64.4
psbK-psbI_p2	psbK-psbI	2	CGTCGATGTTATGCCAGTCA	CGTCCTGGATCATTAGAGAGG	20	21	50.0	52.4	58.1	57.1	0.0	0.0	2.011301	8325	8719	563-565	1.000000	64.2
psbK-psbI_p3	psbK-psbI	3	TCGTCGATGTTATGCCAGTC	CGTCCTGGATCATTAGAGAGG	20	21	50.0	52.4	57.8	57.1	4.2	0.0	2.136345	8325	8719	564-566	1.000000	63.2
psbK-psbI_p4	psbK-psbI	4	TGGCAAGCTGCTGTAAGTTT	CGTCCTGGATCATTAGAGAGG	20	21	45.0	52.4	58.3	57.1	1.4	0.0	2.241348	8325	8719	511-513	1.000000	62.4
psbK-psbI_p5	psbK-psbI	5	TTGGCAAGCTGCTGTAAGTT	CGTCCTGGATCATTAGAGAGG	20	21	45.0	52.4	58.3	57.1	1.4	0.0	2.241348	8325	8719	512-514	1.000000	62.4
trnS-GCU-trnT-CGU_p1	trnS-GCU-trnT-CGU	1	ATTAGCAATCCGCCGCTTTA	GCAAACCGAAAGACCCCTTA	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.137961	9047	9776	845-846	1.000000	79.1
trnS-GCU-trnT-CGU_p2	trnS-GCU-trnT-CGU	2	ACGGAAAGAGAGGGATTCGA	GCAAACCGAAAGACCCCTTA	20	20	50.0	50.0	58.2	58.1	0.0	0.0	0.251103	9047	9776	894-895	1.000000	78.2
trnS-GCU-trnT-CGU_p3	trnS-GCU-trnT-CGU	3	CGAAAACCTCGTACAACGGA	GCAAACCGAAAGACCCCTTA	20	20	50.0	50.0	58.2	58.1	0.0	0.0	0.330022	9047	9776	864-865	1.000000	77.5
trnS-GCU-trnT-CGU_p4	trnS-GCU-trnT-CGU	4	GCTTTAGTCCACTCAGCCAT	GCAAACCGAAAGACCCCTTA	20	20	50.0	50.0	57.6	58.1	0.9	0.0	0.509703	9047	9776	831-832	1.000000	76.1
trnS-GCU-trnT-CGU_p5	trnS-GCU-trnT-CGU	5	AGTCCACTCAGCCATCTCTC	GCAAACCGAAAGACCCCTTA	20	20	55.0	50.0	58.5	58.1	0.0	0.0	0.615716	9047	9776	826-827	1.000000	75.3
rps2_p1	rps2	1	AAGCCAAAGCAACAGTCGTA	TTACCCGGCGAACTAATTGG	20	20	45.0	50.0	58.0	58.0	0.0	9.3	0.065210	16675	17385	1990-1991	1.000000	79.6
rps2_p2	rps2	2	ATGGACTTGGAAACCCCCTA	TTACCCGGCGAACTAATTGG	20	20	50.0	50.0	57.9	58.0	0.0	9.3	0.095835	16675	17385	1674-1675	1.000000	79.4
rps2_p3	rps2	3	AAGCCAAAGCAACAGTCGTA	AATGTCTACGGCCAATCGAC	20	20	45.0	50.0	58.0	58.1	0.0	2.2	0.102274	16675	17385	1455-1455	1.000000	79.3
rps2_p4	rps2	4	ATGGACTTGGAAACCCCCTA	AATGTCTACGGCCAATCGAC	20	20	50.0	50.0	57.9	58.1	0.0	2.2	0.132899	16675	17385	1139-1139	1.000000	79.1
rps2_p5	rps2	5	AAGCCAAAGCAACAGTCGTA	AGGCCTGAAAGAGAACGTTG	20	20	45.0	50.0	58.0	58.1	0.0	3.1	0.153971	16675	17385	1790-1791	1.000000	78.9
rpoC2_p1	rpoC2	1	GCGAAGGGGAAACTAGACTG	AGTGATTGCTGAGATTCGCG	20	20	55.0	50.0	58.0	58.7	0.0	0.0	0.726883	20344	20463	218-218	1.000000	74.9
rpoC2_p2	rpoC2	2	AGCGAAGGGGAAACTAGACT	AGTGATTGCTGAGATTCGCG	20	20	50.0	50.0	58.1	58.7	0.0	0.0	0.775363	20344	20463	219-219	1.000000	74.5
rpoC2_p3	rpoC2	3	CTAGACTGGATCTGCATGGC	AGTGATTGCTGAGATTCGCG	20	20	55.0	50.0	57.8	58.7	0.0	0.0	0.882165	20344	20463	206-206	1.000000	73.7
rpoC2_p4	rpoC2	4	TAGACTGGATCTGCATGGCT	AGTGATTGCTGAGATTCGCG	20	20	50.0	50.0	58.2	58.7	0.0	0.0	0.916717	20344	20463	205-205	1.000000	73.4
rpoC2_p5	rpoC2	5	GAGCGAAGGGGAAACTAGAC	AGTGATTGCTGAGATTCGCG	20	20	55.0	50.0	57.7	58.7	0.0	0.0	1.012174	20344	20463	220-220	1.000000	72.6
trnD-GUC_p1	trnD-GUC	1	AGTAAGGATCTCCCCTTGGG	TTCGCCAATCTACCATGAGA	20	20	55.0	45.0	57.8	56.3	5.5	0.0	1.861554	32238	32311	279-279	1.000000	65.7
trnD-GUC_p2	trnD-GUC	2	AGTAAGGATCTCCCCTTGGG	TCGCCAATCTACCATGAGAT	20	20	55.0	45.0	57.8	56.1	5.5	0.0	2.095009	32238	32311	278-278	1.000000	63.8
trnD-GUC_p3	trnD-GUC	3	TGAAATTCTGCTCCCTGTCC	TTCGCCAATCTACCATGAGA	20	20	50.0	45.0	57.5	56.3	0.0	0.0	2.160118	32238	32311	257-257	1.000000	63.3
trnD-GUC_p4	trnD-GUC	4	AGTAAGGATCTCCCCTTGGG	CCAAGAATTCGCCAATCTACC	20	21	55.0	47.6	57.8	57.0	5.5	0.0	2.169366	32238	32311	286-286	1.000000	63.2
trnD-GUC_p5	trnD-GUC	5	CCTGTCCTCCCTTTCACAGA	TTCGCCAATCTACCATGAGA	20	20	55.0	45.0	58.6	56.3	0.0	0.0	2.311137	32238	32311	244-244	1.000000	62.2
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	57.8	58.0	0.0	0.0	0.165150	32655	33632	1105-1142	1.000000	80.1
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	TTTCGTAGTACCCTACCCCC	CCATGGCGTTACTCTACCAC	20	20	55.0	55.0	57.8	58.1	0.0	0.0	0.223247	32655	33632	1084-1121	1.000000	79.7
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	TTTCGTAGTACCCTACCCCC	TGGCGTTACTCTACCACTGA	20	20	55.0	50.0	57.8	58.1	0.0	0.0	0.245401	32655	33632	1081-1118	1.000000	79.5
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.3	58.0	0.0	0.0	0.269359	32655	33632	1067-1104	1.000000	79.3
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.287509	32655	33632	1046-1083	1.000000	79.2
trnG-GCC-trnM-CAU_p1	trnG-GCC-trnM-CAU	1	ATGCGGATATGGTCGAATGG	GGGTAGAGCAGTTTGGTAGC	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.124730	38760	38941	323-325	1.000000	79.5
trnG-GCC-trnM-CAU_p2	trnG-GCC-trnM-CAU	2	ATGCGGATATGGTCGAATGG	CTTGAGGTCACGGGTTCAAA	20	20	50.0	50.0	57.9	58.0	0.0	4.2	0.137348	38760	38941	287-289	1.000000	79.5
trnG-GCC-trnM-CAU_p3	trnG-GCC-trnM-CAU	3	ATGCGGATATGGTCGAATGG	TCAGTTCTTCTCTTCAGCGC	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.170830	38760	38941	344-346	1.000000	79.1
trnG-GCC-trnM-CAU_p4	trnG-GCC-trnM-CAU	4	TCTTTGCCAAGGAGAAGACG	GGGTAGAGCAGTTTGGTAGC	20	20	50.0	55.0	57.8	58.0	0.0	0.0	0.195421	38760	38941	294-296	1.000000	79.0
trnG-GCC-trnM-CAU_p5	trnG-GCC-trnM-CAU	5	ATGCGGATATGGTCGAATGG	GTCACGGGTTCAAATCCTGT	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.206932	38760	38941	281-283	1.000000	78.9
ycf3-trnS-GGA_p1	ycf3-trnS-GGA	1	TTCTCCTGAAGTTGTCGGAA	CCCTCGGTAAACAAAAGCCT	20	20	45.0	50.0	56.4	58.1	0.0	0.0	1.678533	46784	47664	1042-1044	1.000000	66.8
ycf3-trnS-GGA_p2	ycf3-trnS-GGA	2	TTTCTCCTGAAGTTGTCGGA	CCCTCGGTAAACAAAAGCCT	20	20	45.0	50.0	56.4	58.1	0.0	0.0	1.678533	46784	47664	1043-1045	1.000000	66.8
ycf3-trnS-GGA_p3	ycf3-trnS-GGA	3	TTCTCCTGAAGTTGTCGGAA	ACGGAAAGAGAGGGATTCGA	20	20	45.0	50.0	56.4	58.2	0.0	0.0	1.735519	46784	47664	1063-1065	1.000000	66.3
ycf3-trnS-GGA_p4	ycf3-trnS-GGA	4	TTTCTCCTGAAGTTGTCGGA	ACGGAAAGAGAGGGATTCGA	20	20	45.0	50.0	56.4	58.2	0.0	0.0	1.735519	46784	47664	1064-1066	1.000000	66.3
ycf3-trnS-GGA_p5	ycf3-trnS-GGA	5	TTCTCCTGAAGTTGTCGGAA	TTCCAATGCTACGCCTTGAA	20	20	45.0	45.0	56.4	57.8	0.0	5.6	1.776834	46784	47664	1013-1015	1.000000	66.0
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	20	20	55.0	50.0	58.0	58.1	10.3	0.0	0.089643	51061	51734	816-821	1.000000	79.5
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.139034	51061	51734	780-785	1.000000	79.1
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	GGGATAGCTCAGCTGGTAGA	ATCATCCACGCTTGAAACGT	20	20	55.0	45.0	58.0	58.2	10.3	0.0	0.194095	51061	51734	851-856	1.000000	78.6
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	CTGGTAGAGCAGAGGACTGA	AGTTGGATAGGATGGCCCTT	20	20	55.0	50.0	57.9	58.1	2.5	0.0	0.223436	51061	51734	804-809	1.000000	78.4
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	CCTCGTGTCACCAGTTCAAA	ATCATCCACGCTTGAAACGT	20	20	50.0	45.0	58.1	58.2	0.0	0.0	0.243485	51061	51734	815-820	1.000000	78.2
psbE_p1	psbE	1	ATCTATGGTCATTGGGGCCT	ATCTTTCGCGGACTCAATCC	20	20	50.0	50.0	58.2	58.1	0.8	6.1	0.220064	67556	67807	327-327	1.000000	78.2
psbE_p2	psbE	2	CGATCTATGGTCATTGGGGC	ATCTTTCGCGGACTCAATCC	20	20	55.0	50.0	58.2	58.1	0.0	6.1	0.245162	67556	67807	329-329	1.000000	78.0
psbE_p3	psbE	3	GATCTATGGTCATTGGGGCC	ATCTTTCGCGGACTCAATCC	20	20	55.0	50.0	57.8	58.1	0.0	6.1	0.265251	67556	67807	328-328	1.000000	77.9
psbE_p4	psbE	4	ATCTATGGTCATTGGGGCCT	GGACTCAATCCCCCTTTGAC	20	20	50.0	55.0	58.2	57.9	0.8	0.0	0.291661	67556	67807	318-318	1.000000	77.7
psbE_p5	psbE	5	CGATCTATGGTCATTGGGGC	GGACTCAATCCCCCTTTGAC	20	20	55.0	55.0	58.2	57.9	0.0	0.0	0.316759	67556	67807	320-320	1.000000	77.5
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	GCCGCTAGTAGAAAACCGAA	20	20	50.0	50.0	57.9	58.0	6.4	0.0	0.051764	67808	69092	1430-1431	1.000000	80.0
psbE-petL_p2	psbE-petL	2	CAACCCGCAATGAATAGGGA	AAGCCGCTAGTAGAAAACCG	20	20	50.0	50.0	57.9	58.0	6.4	0.0	0.057305	67808	69092	1432-1433	1.000000	79.9
psbE-petL_p3	psbE-petL	3	AGAACGTTCTCCTGTGCTTC	GCCGCTAGTAGAAAACCGAA	20	20	50.0	50.0	57.8	58.0	24.9	0.0	0.156772	67808	69092	1353-1354	1.000000	79.2
psbE-petL_p4	psbE-petL	4	AGAACGTTCTCCTGTGCTTC	AAGCCGCTAGTAGAAAACCG	20	20	50.0	50.0	57.8	58.0	24.9	0.0	0.162314	67808	69092	1355-1356	1.000000	79.1
psbE-petL_p5	psbE-petL	5	ACGAATAACCAACCCGCAAT	GCCGCTAGTAGAAAACCGAA	20	20	45.0	50.0	58.2	58.0	0.0	0.0	0.171450	67808	69092	1439-1440	1.000000	79.0
petL-petG_p1	petL-petG	1	TTCGGTTTTCTACTAGCGGC	AGCGCTCTTAGTTCAGTTCG	20	20	50.0	50.0	58.0	58.0	0.0	2.6	0.016457	69189	69362	568-571	1.000000	83.8
petL-petG_p2	petL-petG	2	CGGTTTTCTACTAGCGGCTT	AGCGCTCTTAGTTCAGTTCG	20	20	50.0	50.0	58.0	58.0	8.3	2.6	0.021998	69189	69362	566-569	1.000000	83.8
petL-petG_p3	petL-petG	3	TTCGGTTTTCTACTAGCGGC	AGAACGTGGGTCTCCAAAAC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.037257	69189	69362	546-549	1.000000	83.8
petL-petG_p4	petL-petG	4	TTCGGTTTTCTACTAGCGGC	GGATTCCGTTCCCGATTTCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.033110	69189	69362	973-976	1.000000	82.1
petL-petG_p5	petL-petG	5	TTCGGTTTTCTACTAGCGGC	TGGATTCCGTTCCCGATTTC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.033110	69189	69362	974-977	1.000000	82.1
ndhD_p1	ndhD	1	CGTAAGCGAAGAAAGAGCCT	TACCGATAAAACCCGTGCTC	20	20	50.0	50.0	58.0	57.7	0.0	0.0	0.300742	119648	121150	1691-1691	1.000000	77.7
ndhD_p2	ndhD	2	CGTAAGCGAAGAAAGAGCCT	ACGGGTTTTTCTGGTCCAAG	20	20	50.0	50.0	58.0	58.3	0.0	0.0	0.314768	119648	121150	1640-1640	1.000000	77.6
ndhD_p3	ndhD	3	CGTAAGCGAAGAAAGAGCCT	CGGGTTTTTCTGGTCCAAGT	20	20	50.0	50.0	58.0	58.3	0.0	0.0	0.314768	119648	121150	1639-1639	1.000000	77.6
ndhD_p4	ndhD	4	CGTAAGCGAAGAAAGAGCCT	TTTCGAGCACGGGTTTTTCT	20	20	50.0	45.0	58.0	58.3	0.0	3.1	0.331540	119648	121150	1648-1648	1.000000	77.4
ndhD_p5	ndhD	5	CGTAAGCGAAGAAAGAGCCT	TTTTCGAGCACGGGTTTTTC	20	20	50.0	45.0	58.0	57.5	0.0	3.1	0.480878	119648	121150	1649-1649	1.000000	76.3
ndhE_p1	ndhE	1	ATCCTACCTTACGCGACAGA	GTTGGGGATGGGGTTACTTC	20	20	50.0	55.0	58.0	57.9	15.9	0.0	0.187767	121793	122098	853-853	1.000000	78.7
ndhE_p2	ndhE	2	GACCCATGCTACGAGTTGTT	GTTGGGGATGGGGTTACTTC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.234086	121793	122098	1274-1274	1.000000	78.4
ndhE_p3	ndhE	3	ACATCCGTCCCAAGGTATCA	GTTGGGGATGGGGTTACTTC	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.265194	121793	122098	1141-1141	1.000000	78.2
ndhE_p4	ndhE	4	ACTTCTGGATTGGGATTGGC	GTTGGGGATGGGGTTACTTC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.289296	121793	122098	923-923	1.000000	77.9
ndhE_p5	ndhE	5	ACCCGCAGATATTGGCAAAA	GTTGGGGATGGGGTTACTTC	20	20	45.0	55.0	58.2	57.9	3.0	0.0	0.293317	121793	122098	1469-1469	1.000000	77.9
ycf1_p1	ycf1	1	ATGCTTGTTCTAGCGGTCAG	AACAGCCTCAAACGAAACAA	20	20	50.0	40.0	58.0	56.4	0.0	0.0	1.619652	130389	130538	255-255	1.000000	68.3
ycf1_p2	ycf1	2	ATGCTTGTTCTAGCGGTCAG	ACAGCCTCAAACGAAACAAA	20	20	50.0	40.0	58.0	56.4	0.0	0.0	1.619652	130389	130538	254-254	1.000000	68.3
ycf1_p3	ycf1	3	TGTTCTAGCGGTCAGATCCT	AACAGCCTCAAACGAAACAA	20	20	50.0	40.0	57.9	56.4	0.0	0.0	1.749313	130389	130538	250-250	1.000000	67.3
ycf1_p4	ycf1	4	TGTTCTAGCGGTCAGATCCT	ACAGCCTCAAACGAAACAAA	20	20	50.0	40.0	57.9	56.4	0.0	0.0	1.749313	130389	130538	249-249	1.000000	67.3
ycf1_p5	ycf1	5	ATGCTTGTTCTAGCGGTCAG	ACAGCCTCAAACGAAACAAAA	20	21	50.0	38.1	58.0	57.1	0.0	0.0	1.875147	130389	130538	254-254	1.000000	66.3