Hotspot

rpoC1

LSC · 23909-26807 · 2899 bp

Back to Hellenia

Pi 0.00104

MarkerSeek score 57.20

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2899
variable_sites: 3
pi: 0.00104
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	GCACGAACATCGCCTAATCT	AGGAAGGAAGCTTGATCTGA	50.0	45.0	58.4	55.8	2971-2993 bp	1.0	60.0
rpoC1_p2	GCACGAACATCGCCTAATCT	AGGAAGGAAGCTTGATCTGAA	50.0	42.9	58.4	56.6	2971-2993 bp	1.0	58.3
rpoC1_p3	GCACGAACATCGCCTAATCT	AGGAAGGAAGCTTGATCTGAAT	50.0	40.9	58.4	57.1	2971-2993 bp	1.0	54.4
rpoC1_p4	GCACGAACATCGCCTAATCT	GGAAGGAAGCTTGATCTGAATG	50.0	45.5	58.4	57.1	2970-2992 bp	1.0	54.1
rpoC1_p5	GCACGAACATCGCCTAATCT	GGAAGGAAGCTTGATCTGAA	50.0	45.0	58.4	55.0	2970-2992 bp	1.0	53.6