Hotspot

rpl16

LSC · 89120-91354 · 2235 bp

Back to Hellenia

Pi 0.00378
MarkerSeek score 64.10
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
2235
variable_sites
8
pi
0.00378
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 GCGGTTGTTTTTCATCCTCG CGATTGACCATTTGTTAGAATTGC 50.0 37.5 58.0 57.4 2202-2287 bp 1.0 47.5
rpl16_p2 GCGGTTGTTTTTCATCCTCG TGACCATTTGTTAGAATTGCT 50.0 33.3 58.0 53.7 2198-2283 bp 1.0 44.3
rpl16_p3 GCGGTTGTTTTTCATCCTCG TTGACCATTTGTTAGAATTGCT 50.0 31.8 58.0 54.5 2199-2284 bp 1.0 44.3
rpl16_p4 TGCGGTTGTTTTTCATCCTC CGATTGACCATTTGTTAGAATTGC 45.0 37.5 56.9 57.4 2203-2288 bp 1.0 44.3
rpl16_p5 GCGGTTGTTTTTCATCCTCG CGATTGACCATTTGTTAGAATTGCT 50.0 36.0 58.0 58.7 2202-2287 bp 1.0 44.3