Hotspot

psaC-ndhE

SSC · 128121-129373 · 1253 bp

Back to Hellenia

Pi 0.000834

MarkerSeek score 63.40

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 1253
variable_sites: 1
pi: 0.000834
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	ATACATCTGTAGGGCAGGCT	CTGAAGCAGCTATTGGGCTA	50.0	50.0	57.9	57.4	1367-1421 bp	1.0	77.3
psaC-ndhE_p2	ATACATCTGTAGGGCAGGCT	TGAAGCAGCTATTGGGCTAG	50.0	50.0	57.9	57.4	1366-1420 bp	1.0	77.3
psaC-ndhE_p3	ACATCCGTCCCAAGGTATCA	CTGAAGCAGCTATTGGGCTA	50.0	50.0	58.1	57.4	1393-1447 bp	1.0	77.1
psaC-ndhE_p4	ACATCCGTCCCAAGGTATCA	TGAAGCAGCTATTGGGCTAG	50.0	50.0	58.1	57.4	1392-1446 bp	1.0	77.1
psaC-ndhE_p5	ATACATCTGTAGGGCAGGCT	GAAGCAGCTATTGGGCTAGC	50.0	55.0	57.9	58.8	1365-1419 bp	1.0	76.2