Hotspot

ccsA-ndhD

SSC · 125318-126233 · 916 bp

Back to Hellenia

Pi 0.00851

MarkerSeek score 66.90

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 916
variable_sites: 7
pi: 0.00851
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	ATTCAGCACTTGTGGCTTCT	GTTGACAGGGCCCAAGTTAT	45.0	50.0	57.7	57.8	984-1077 bp	1.0	80.6
ccsA-ndhD_p2	ATTCAGCACTTGTGGCTTCT	CTCGCTATCAGTTGACAGGG	45.0	55.0	57.7	57.8	994-1087 bp	1.0	80.5
ccsA-ndhD_p3	ATTCAGCACTTGTGGCTTCT	CCGATTTCGTTCTCTCGCTA	45.0	50.0	57.7	57.5	1007-1100 bp	1.0	78.5
ccsA-ndhD_p4	ATTCAGCACTTGTGGCTTCT	ATCCCGATTTCGTTCTCTCG	45.0	50.0	57.7	57.5	1010-1103 bp	1.0	78.3
ccsA-ndhD_p5	ATTCAGCACTTGTGGCTTCT	TATCAGTTGACAGGGCCCAA	45.0	50.0	57.7	58.6	989-1082 bp	1.0	77.2