Hotspot

rpl32-trnL-UAG

SSC · 123273-124164 · 892 bp

Back to Hellenia

Pi 0
MarkerSeek score 62.90
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
892
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 CCGGGCGTTCAAAAAGTTTT AGCGTGTCTACCGATTTCAC 45.0 50.0 58.1 58.0 929-977 bp 1.0 83.4
rpl32-trnL-UAG_p2 CCGGGCGTTCAAAAAGTTTT CAGCGTGTCTACCGATTTCA 45.0 50.0 58.1 58.0 930-978 bp 1.0 83.4
rpl32-trnL-UAG_p3 CCGGGCGTTCAAAAAGTTTT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.1 57.9 939-987 bp 1.0 82.4
rpl32-trnL-UAG_p4 CCGGGCGTTCAAAAAGTTTT CCTAAGAGCAGCGTGTCTAC 45.0 55.0 58.1 57.8 938-986 bp 1.0 81.9
rpl32-trnL-UAG_p5 CCGGGCGTTCAAAAAGTTTT CTAAGAGCAGCGTGTCTACC 45.0 55.0 58.1 57.8 937-985 bp 1.0 81.9