Hotspot

ndhF-rpl32

SSC · 122165-123098 · 934 bp

Back to Hellenia

Pi 0.00216

MarkerSeek score 60.60

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 934
variable_sites: 2
pi: 0.00216
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCTGTCGGAATCGGAAT	GAAAAAGCCTTTACTGCGGC	50.0	50.0	58.6	58.3	1130-1136 bp	1.0	73.1
ndhF-rpl32_p2	TTTTGTTGCTGTCGGAATCG	GAAAAAGCCTTTACTGCGGC	45.0	50.0	57.3	58.3	1134-1140 bp	1.0	72.7
ndhF-rpl32_p3	TGCTGTCGGAATCGGAATAA	GAAAAAGCCTTTACTGCGGC	45.0	50.0	57.0	58.3	1128-1134 bp	1.0	70.4
ndhF-rpl32_p4	TTGCTGTCGGAATCGGAATA	GAAAAAGCCTTTACTGCGGC	45.0	50.0	57.0	58.3	1129-1135 bp	1.0	70.4
ndhF-rpl32_p5	TTTTTGTTGCTGTCGGAATCG	GAAAAAGCCTTTACTGCGGC	42.9	50.0	58.0	58.3	1135-1141 bp	1.0	70.1