Hotspot

rbcL-accD

LSC · 63218-64037 · 820 bp

Back to Hellenia

Pi 0

MarkerSeek score 60.20

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 820
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GCAAATGGAGCCCTGAACTA	CAACATCGAATTGAACCGCC	50.0	50.0	57.9	58.1	936-948 bp	1.0	79.1
rbcL-accD_p2	TAGCAAATGGAGCCCTGAAC	CAACATCGAATTGAACCGCC	50.0	50.0	57.9	58.1	938-950 bp	1.0	79.1
rbcL-accD_p3	TGAAGCTAGCAAATGGAGCC	CAACATCGAATTGAACCGCC	50.0	50.0	58.2	58.1	944-956 bp	1.0	78.2
rbcL-accD_p4	CCGTGAAGCTAGCAAATGGA	CAACATCGAATTGAACCGCC	50.0	50.0	58.3	58.1	947-959 bp	1.0	78.1
rbcL-accD_p5	TCCGTGAAGCTAGCAAATGG	CAACATCGAATTGAACCGCC	50.0	50.0	58.3	58.1	948-960 bp	1.0	78.1