Hotspot

atpB-rbcL

LSC · 60925-61753 · 829 bp

Back to Hellenia

Pi 0.00241

MarkerSeek score 60.10

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 829
variable_sites: 2
pi: 0.00241
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	GGTACGCCCTAGGTTTTGTT	GGTGACATAAGTCCCTCCCT	50.0	55.0	57.8	58.1	927 bp	1.0	77.7
atpB-rbcL_p2	GGTACGCCCTAGGTTTTGTT	TGGTGACATAAGTCCCTCCC	50.0	55.0	57.8	58.4	928 bp	1.0	75.3
atpB-rbcL_p3	CTGGGCCAATAATTTGAGCG	GGTGACATAAGTCCCTCCCT	50.0	55.0	57.5	58.1	946 bp	1.0	75.2
atpB-rbcL_p4	GCAGGACTAGAAGGGGTAGG	GGTGACATAAGTCCCTCCCT	60.0	55.0	58.6	58.1	890 bp	1.0	74.5
atpB-rbcL_p5	ACGCCCTAGGTTTTGTTCTT	GGTGACATAAGTCCCTCCCT	45.0	55.0	57.3	58.1	924 bp	1.0	74.0