Hotspot

trnF-GAA-ndhJ

LSC · 53366-54120 · 755 bp

Back to Hellenia

Pi 0.00397

MarkerSeek score 48.50

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 755
variable_sites: 3
pi: 0.00397
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	CCCAATTTCTATGAAATACAAGATGC	50.0	34.6	58.1	57.2	822 bp	1.0	40.3
trnF-GAA-ndhJ_p2	GGGAAATGAAATGGTCGGGA	CCCAATTTCTATGAAATACAAGATGC	50.0	34.6	57.9	57.2	874 bp	1.0	40.3
trnF-GAA-ndhJ_p3	AATGGTCGGGATAGCTCAGT	CCCAATTTCTATGAAATACAAGATGC	50.0	34.6	58.2	57.2	865 bp	1.0	40.3
trnF-GAA-ndhJ_p4	ATGGTCGGGATAGCTCAGTT	CCCAATTTCTATGAAATACAAGATGC	50.0	34.6	58.2	57.2	864 bp	1.0	40.3
trnF-GAA-ndhJ_p5	GCACGGGAAATGAAATGGTC	CCCAATTTCTATGAAATACAAGATGC	50.0	34.6	57.7	57.2	878 bp	1.0	40.3