Hotspot

trnC-GCA-petN

LSC · 31384-32510 · 1127 bp

Back to Hellenia

Pi 0.00177
MarkerSeek score 59.80
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
1127
variable_sites
2
pi
0.00177
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 GATTTGGTTTGGCGACATGG TACCCCTAGAGTCCACTCCT 50.0 55.0 58.0 58.0 1303 bp 1.0 80.1
trnC-GCA-petN_p2 GGATTTGGTTTGGCGACATG TACCCCTAGAGTCCACTCCT 50.0 55.0 58.0 58.0 1304 bp 1.0 80.1
trnC-GCA-petN_p3 GATTTGGTTTGGCGACATGG GTACCCCTAGAGTCCACTCC 50.0 60.0 58.0 57.9 1304 bp 1.0 79.5
trnC-GCA-petN_p4 GGATTTGGTTTGGCGACATG GTACCCCTAGAGTCCACTCC 50.0 60.0 58.0 57.9 1305 bp 1.0 79.5
trnC-GCA-petN_p5 GATTTGGTTTGGCGACATGG GTCCACTCCTTCCCCATACT 50.0 55.0 58.0 58.1 1293 bp 1.0 79.1