Hotspot

atpH-atpI

LSC · 16119-17641 · 1523 bp

Back to Hellenia

Pi 0.00136

MarkerSeek score 62.80

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1523
variable_sites: 2
pi: 0.00136
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	TACCTTCTACAGCTTGGCCT	ATAGGGGAATCCATGGAGGG	50.0	55.0	58.0	57.9	1616-1668 bp	1.0	81.7
atpH-atpI_p2	TACCTTCTACAGCTTGGCCT	ATTTTTGCAACCTTAGCCGC	50.0	45.0	58.0	57.9	1643-1695 bp	1.0	81.6
atpH-atpI_p3	AACAGAAGCGGCAGAAATCA	ATAGGGGAATCCATGGAGGG	45.0	55.0	58.1	57.9	1531-1583 bp	1.0	81.4
atpH-atpI_p4	AACAGAAGCGGCAGAAATCA	ATTTTTGCAACCTTAGCCGC	45.0	45.0	58.1	57.9	1558-1610 bp	1.0	81.3
atpH-atpI_p5	CAGCAGCAATAACAGAAGCG	ATAGGGGAATCCATGGAGGG	50.0	55.0	57.8	57.9	1541-1593 bp	1.0	80.9