Hotspot

atpF-atpH

LSC · 15344-15872 · 529 bp

Back to Hellenia

Pi 0.00195

MarkerSeek score 61.10

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 529
variable_sites: 1
pi: 0.00195
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	ATTAAACCCGAAACTCCCGG	AATTTACGGACTGGTCGTGG	50.0	50.0	57.9	57.9	638-653 bp	1.0	80.3
atpF-atpH_p2	AATGGCCCAAGGAAACGAAA	AATTTACGGACTGGTCGTGG	45.0	50.0	58.3	57.9	609-624 bp	1.0	79.1
atpF-atpH_p3	ATTAAACCCGAAACTCCCGG	TCGTGGCATTAGCGCTTTTA	50.0	45.0	57.9	58.3	624-639 bp	1.0	78.9
atpF-atpH_p4	AATGGCCCAAGGAAACGAAA	TCGTGGCATTAGCGCTTTTA	45.0	45.0	58.3	58.3	595-610 bp	1.0	77.6
atpF-atpH_p5	GGCCCAAGGAAACGAAAGAA	AATTTACGGACTGGTCGTGG	50.0	50.0	58.7	57.9	606-621 bp	1.0	76.0