Hotspot

trnS-GCU-trnG-UCC

LSC · 9620-11295 · 1676 bp

Back to Hellenia

Pi 0.00307
MarkerSeek score 62.90
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
1676
variable_sites
5
pi
0.00307
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 TTAGCAATCCGACGCCTTAG AACTGAGAGACCCCTTAACT 50.0 45.0 57.8 55.1 1776-1787 bp 1.0 57.1
trnS-GCU-trnG-UCC_p2 TTAGCAATCCGACGCCTTAG ACTGAGAGACCCCTTAACTT 50.0 45.0 57.8 55.1 1775-1786 bp 1.0 57.1
trnS-GCU-trnG-UCC_p3 TTAGCAATCCGACGCCTTAG ACTGAGAGACCCCTTAACTTTTG 50.0 43.5 57.8 58.0 1775-1786 bp 1.0 56.4
trnS-GCU-trnG-UCC_p4 CGGAGAGAGAGGGATTCGAA AACTGAGAGACCCCTTAACT 55.0 45.0 58.3 55.1 1825-1836 bp 1.0 56.3
trnS-GCU-trnG-UCC_p5 CGGAGAGAGAGGGATTCGAA ACTGAGAGACCCCTTAACTT 55.0 45.0 58.3 55.1 1824-1835 bp 1.0 56.3