Hotspot

petD

LSC · 57733-58949 · 1217 bp

Back to Helleborus

Pi 0.0358

MarkerSeek score 75.20

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1217
variable_sites: 124
pi: 0.0358
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	ACCCCTTCTTACTGCCGTAT	CCCAGAGACATACGTTGTGG	50.0	55.0	58.1	58.0	1526-1552 bp	1.0	87.4
petD_p2	ACCCCTTCTTACTGCCGTAT	CCCCAGAGACATACGTTGTG	50.0	55.0	58.1	58.0	1527-1553 bp	1.0	87.4
petD_p3	ACGGGATTTGCCATGACTTT	CCCAGAGACATACGTTGTGG	45.0	55.0	58.1	58.0	1951-1977 bp	1.0	86.2
petD_p4	ACGGGATTTGCCATGACTTT	CCCCAGAGACATACGTTGTG	45.0	55.0	58.1	58.0	1952-1978 bp	1.0	86.2
petD_p5	ACGGGATTTGCCATGACTTT	TCCCCAGAGACATACGTTGT	45.0	50.0	58.1	58.1	1953-1979 bp	1.0	85.7