Hotspot

ycf2-trnI-CAU

IRa · 113338-115746 · 2409 bp

Back to Helleborus

Pi 0.0782
MarkerSeek score 80.00
Primer available yes
Label ycf2-trnI-CAU

Feature Metadata

label_name
ycf2-trnI-CAU
feature_type
igs
region
IRa
length_bp
2409
variable_sites
458
pi
0.0782
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf2-trnI-CAU_p1 TCGTTCCAAGCTCGAAGTAC GTTGGGCGCTTTAACCATTC 50.0 50.0 57.7 58.0 2472 bp 0.142857 42.9
ycf2-trnI-CAU_p2 GTTCCAAGCTCGAAGTACCA GTTGGGCGCTTTAACCATTC 50.0 50.0 57.5 58.0 2470 bp 0.142857 42.0
ycf2-trnI-CAU_p3 CTTCATGCTCGTTCCAAGCT GTTGGGCGCTTTAACCATTC 50.0 50.0 58.6 58.0 2480 bp 0.142857 41.2
ycf2-trnI-CAU_p4 TCTTCATGCTCGTTCCAAGC GTTGGGCGCTTTAACCATTC 50.0 50.0 58.6 58.0 2481 bp 0.142857 41.2
ycf2-trnI-CAU_p5 TTCATGCTCGTTCCAAGCTC GTTGGGCGCTTTAACCATTC 50.0 50.0 58.6 58.0 2479 bp 0.142857 41.2