Hotspot

trnV-GAC-rps7

IRa · 107176-109638 · 2463 bp

Back to Helleborus

Pi 0.0837

MarkerSeek score 74.70

Primer available yes

Label trnV-GAC-rps7

Feature Metadata

label_name: trnV-GAC-rps7
feature_type: igs
region: IRa
length_bp: 2463
variable_sites: 316
pi: 0.0837
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 74.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-GAC-rps7_p1	TCGTAGACAAAGCGGATTCG	AACTTGATGAGTCGATCCGC	50.0	50.0	58.1	58.1	1760-2896 bp	0.571429	70.2
trnV-GAC-rps7_p2	TCGTAGACAAAGCGGATTCG	CATATTTCGACCCGGACGTT	50.0	50.0	58.1	58.1	1847-2983 bp	0.571429	70.0
trnV-GAC-rps7_p3	TCCATGCGCTTCAAGAAACT	GCAGCATCCACTAATTCGGA	45.0	50.0	58.1	58.0	2966 bp	0.142857	44.5
trnV-GAC-rps7_p4	TCCATGCGCTTCAAGAAACT	CATATTTCGACCCGGACGTT	45.0	50.0	58.1	58.1	2931 bp	0.142857	44.3
trnV-GAC-rps7_p5	TCCATGCGCTTCAAGAAACT	AACTTGATGAGTCGATCCGC	45.0	50.0	58.1	58.1	2844 bp	0.142857	44.3