Hotspot

trnN-GUU-trnR-ACG

SSC · 98802-99399 · 598 bp

Back to Helleborus

Pi 0.0506

MarkerSeek score 75.40

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: SSC
length_bp: 598
variable_sites: 71
pi: 0.0506
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.71
markerseek_score: 75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	CAGAAATCCGTCGGCTGTTA	CCTTGTTCCTACATGACCCG	50.0	55.0	58.0	58.0	1327 bp	0.142857	45.5
trnN-GUU-trnR-ACG_p2	CAGAAATCCGTCGGCTGTTA	CAATTCGATCAGGCATTCGC	50.0	50.0	58.0	58.0	1305 bp	0.142857	45.5
trnN-GUU-trnR-ACG_p3	ATGTGGTCAGAAATCCGTCG	CCTTGTTCCTACATGACCCG	50.0	55.0	58.0	58.0	1334 bp	0.142857	45.4
trnN-GUU-trnR-ACG_p4	ATGTGGTCAGAAATCCGTCG	CAATTCGATCAGGCATTCGC	50.0	50.0	58.0	58.0	1312 bp	0.142857	45.4
trnN-GUU-trnR-ACG_p5	CAGAAATCCGTCGGCTGTTA	TGAAATGGCTGAGGAGAGGA	50.0	50.0	58.0	58.0	800 bp	0.142857	45.3