Hotspot

ndhF-rpl32

SSC · 87571-88455 · 885 bp

Back to Helleborus

Pi 0.0241

MarkerSeek score 71.00

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 885
variable_sites: 54
pi: 0.0241
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AGGAACTGGAAGGGGAATGA	TTACGAATACGCTTTTTGGA	50.0	35.0	57.9	53.3	752-1178 bp	0.857143	42.4
ndhF-rpl32_p2	GGAGAAGACCCACCCCTATT	TTACGAATACGCTTTTTGGA	55.0	35.0	58.1	53.3	777-1203 bp	0.857143	42.0
ndhF-rpl32_p3	AGGAACTGGAAGGGGAATGA	ACGCTTTTTGGATATAGAAGTACG	50.0	37.5	57.9	57.2	744-1170 bp	0.857143	41.6
ndhF-rpl32_p4	AGGAACTGGAAGGGGAATGA	CGCTTTTTGGATATAGAAGTACGT	50.0	37.5	57.9	57.2	743-1169 bp	0.857143	41.6
ndhF-rpl32_p5	AGGAACTGGAAGGGGAATGA	TTTACGAATACGCTTTTTGGA	50.0	33.3	57.9	54.2	753-1179 bp	0.857143	41.5