Hotspot

rps7-trnV-GAC

IRb · 73444-75928 · 2485 bp

Back to Helleborus

Pi 0.0768

MarkerSeek score 74.20

Primer available yes

Label rps7-trnV-GAC

Feature Metadata

label_name: rps7-trnV-GAC
feature_type: igs
region: IRb
length_bp: 2485
variable_sites: 285
pi: 0.0768
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps7-trnV-GAC_p1	AACTTGATGAGTCGATCCGC	TCGTAGACAAAGCGGATTCG	50.0	50.0	58.1	58.1	1760-2896 bp	0.571429	68.9
rps7-trnV-GAC_p2	CATATTTCGACCCGGACGTT	TCGTAGACAAAGCGGATTCG	50.0	50.0	58.1	58.1	1847-2983 bp	0.571429	68.8
rps7-trnV-GAC_p3	GCAGCATCCACTAATTCGGA	TCCATGCGCTTCAAGAAACT	50.0	45.0	58.0	58.1	2966 bp	0.142857	44.5
rps7-trnV-GAC_p4	CATATTTCGACCCGGACGTT	TCCATGCGCTTCAAGAAACT	50.0	45.0	58.1	58.1	2931 bp	0.142857	44.3
rps7-trnV-GAC_p5	AACTTGATGAGTCGATCCGC	TCCATGCGCTTCAAGAAACT	50.0	45.0	58.1	58.1	2844 bp	0.142857	44.3