Hotspot

psbT-psbN

LSC · 55612-55648 · 37 bp

Back to Helleborus

Pi 0.036

MarkerSeek score 71.90

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 37
variable_sites: 4
pi: 0.036
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	GCCTCCTAAGGTTCCGACTA	GGGTACGCCTTATATACCGC	55.0	55.0	58.2	57.7	140-192 bp	0.714286	67.0
psbT-psbN_p2	GCCTCCTAAGGTTCCGACTA	GATCCATTCGAGGAACACGG	55.0	55.0	58.2	58.4	92-144 bp	0.714286	66.9
psbT-psbN_p3	TTCGAGAGCCTCCTAAGGTT	GGGTACGCCTTATATACCGC	50.0	55.0	57.8	57.7	147-199 bp	0.714286	66.8
psbT-psbN_p4	TTTCGAGAGCCTCCTAAGGT	GGGTACGCCTTATATACCGC	50.0	55.0	57.8	57.7	148-200 bp	0.714286	66.8
psbT-psbN_p5	TTCGAGAGCCTCCTAAGGTT	GATCCATTCGAGGAACACGG	50.0	55.0	57.8	58.4	99-151 bp	0.714286	66.6