Hotspot

trnP-UGG-psaJ

LSC · 49665-50018 · 354 bp

Back to Helleborus

Pi 0.0642

MarkerSeek score 73.40

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 354
variable_sites: 46
pi: 0.0642
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 73.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	TCCAGGTAGCCTATAGTGCC	ACGGGTGCCACAGATAGATA	55.0	50.0	58.0	57.9	988-1139 bp	1.0	91.3
trnP-UGG-psaJ_p2	TCCAGGTAGCCTATAGTGCC	TCGTTTCGGACACAACTGTT	55.0	45.0	58.0	58.0	1577-1731 bp	0.857143	85.8
trnP-UGG-psaJ_p3	CCAGGTAGCCTATAGTGCCT	TCGTTTCGGACACAACTGTT	55.0	45.0	58.0	58.0	1576-1730 bp	0.857143	85.7
trnP-UGG-psaJ_p4	TCCAGGTAGCCTATAGTGCC	CTTTGGCCATGAACCTCCTT	55.0	50.0	58.0	58.1	1522-1676 bp	0.857143	85.6
trnP-UGG-psaJ_p5	CCAGGTAGCCTATAGTGCCT	CTTTGGCCATGAACCTCCTT	55.0	50.0	58.0	58.1	1521-1675 bp	0.857143	85.5