Hotspot

trnS-GGA-rps4

LSC · 30173-30218 · 46 bp

Back to Helleborus

Pi 0.13
MarkerSeek score 73.50
Primer available yes
Label trnS-GGA-rps4

Feature Metadata

label_name
trnS-GGA-rps4
feature_type
igs
region
LSC
length_bp
46
variable_sites
14
pi
0.13
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GGA-rps4_p1 AGGCTTTTGTTTACCGAGGG TGAGCTGCTAGTCGTAGAAT 50.0 45.0 58.1 55.8 126-388 bp 0.428571 42.5
trnS-GGA-rps4_p2 AGGCTTTTGTTTACCGAGGG AATGAGCTGCTAGTCGTAGA 50.0 45.0 58.1 55.8 128-390 bp 0.428571 42.4
trnS-GGA-rps4_p3 AGGCTTTTGTTTACCGAGGG ATGAGCTGCTAGTCGTAGAA 50.0 45.0 58.1 55.8 127-389 bp 0.428571 42.4
trnS-GGA-rps4_p4 AGGCTTTTGTTTACCGAGGG TGACTCTTCACTCATCCCAA 50.0 45.0 58.1 55.8 202-464 bp 0.428571 41.6
trnS-GGA-rps4_p5 AGGCTTTTGTTTACCGAGGG TTGACTCTTCACTCATCCCA 50.0 45.0 58.1 55.8 203-465 bp 0.428571 41.6