Hotspot

ycf1

SSC · 95231-100069 · 4839 bp

Back to Helixanthera

Pi 0.00766

MarkerSeek score 52.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 4839
variable_sites: 37
pi: 0.00766
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTCTGTCGCGCCTTACTTAG	ACCAAAACACTTTTCCGGGA	50.0	45.0	57.7	57.9	1086-1089 bp	1.0	78.5
ycf1_p2	TCTGTCGCGCCTTACTTAGT	ACCAAAACACTTTTCCGGGA	50.0	45.0	58.8	57.9	1085-1088 bp	1.0	74.0
ycf1_p3	CTGTCGCGCCTTACTTAGTA	ACCAAAACACTTTTCCGGGA	50.0	45.0	57.2	57.9	1084-1087 bp	1.0	74.0
ycf1_p4	ATTCTGTCGCGCCTTACTTA	ACCAAAACACTTTTCCGGGA	45.0	45.0	57.0	57.9	1087-1090 bp	1.0	73.0
ycf1_p5	TATTCTGTCGCGCCTTACTT	ACCAAAACACTTTTCCGGGA	45.0	45.0	57.0	57.9	1088-1091 bp	1.0	73.0