Hotspot

trnV-GAC-rps12

IRa · 107266-108541 · 1276 bp

Back to Helixanthera

Pi 0.0223
MarkerSeek score 73.00
Primer available yes
Label trnV-GAC-rps12

Feature Metadata

label_name
trnV-GAC-rps12
feature_type
igs
region
IRa
length_bp
1276
variable_sites
28
pi
0.0223
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnV-GAC-rps12_p1 GATGACTTCCACCACGTCAA TGGCAACTTTACGTAAGGCA 50.0 45.0 57.8 57.7 1372-1593 bp 1.0 79.2
trnV-GAC-rps12_p2 AACTGATGACTTCCACCACG TGGCAACTTTACGTAAGGCA 50.0 45.0 57.8 57.7 1376-1597 bp 1.0 79.2
trnV-GAC-rps12_p3 ATGACTTCCACCACGTCAAG TGGCAACTTTACGTAAGGCA 50.0 45.0 57.8 57.7 1371-1592 bp 1.0 79.2
trnV-GAC-rps12_p4 CTGATGACTTCCACCACGTC TGGCAACTTTACGTAAGGCA 55.0 45.0 58.3 57.7 1374-1595 bp 1.0 78.2
trnV-GAC-rps12_p5 GATGACTTCCACCACGTCAA ACGTAAGGCAGAGTTTGGTT 50.0 45.0 57.8 57.4 1362-1583 bp 1.0 76.2