Hotspot

rps12-trnV-GAC

IRb · 83605-84880 · 1276 bp

Back to Helixanthera

Pi 0.0223
MarkerSeek score 73.00
Primer available yes
Label rps12-trnV-GAC

Feature Metadata

label_name
rps12-trnV-GAC
feature_type
igs
region
IRb
length_bp
1276
variable_sites
28
pi
0.0223
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps12-trnV-GAC_p1 TGGCAACTTTACGTAAGGCA GATGACTTCCACCACGTCAA 45.0 50.0 57.7 57.8 1372-1593 bp 1.0 79.2
rps12-trnV-GAC_p2 TGGCAACTTTACGTAAGGCA AACTGATGACTTCCACCACG 45.0 50.0 57.7 57.8 1376-1597 bp 1.0 79.2
rps12-trnV-GAC_p3 TGGCAACTTTACGTAAGGCA ATGACTTCCACCACGTCAAG 45.0 50.0 57.7 57.8 1371-1592 bp 1.0 79.2
rps12-trnV-GAC_p4 TGGCAACTTTACGTAAGGCA CTGATGACTTCCACCACGTC 45.0 55.0 57.7 58.3 1374-1595 bp 1.0 78.2
rps12-trnV-GAC_p5 ACGTAAGGCAGAGTTTGGTT GATGACTTCCACCACGTCAA 45.0 50.0 57.4 57.8 1362-1583 bp 1.0 76.2