Hotspot

petD-rpoA

LSC · 64758-65020 · 263 bp

Back to Helixanthera

Pi 0.0312

MarkerSeek score 72.90

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 263
variable_sites: 8
pi: 0.0312
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	TACAACAGCCCTTTGGTTGG	GAGCATTTTCGAATAGCAGATGT	50.0	39.1	58.3	57.6	416-423 bp	1.0	53.1
petD-rpoA_p2	CGTCCAATAGCGACAACCAT	GAGCATTTTCGAATAGCAGATGT	50.0	39.1	58.3	57.6	451-458 bp	1.0	52.5
petD-rpoA_p3	TACAACAGCCCTTTGGTTGG	AGCATTTTCGAATAGCAGATGT	50.0	36.4	58.3	56.5	415-422 bp	1.0	52.4
petD-rpoA_p4	CGTCCAATAGCGACAACCAT	AGCATTTTCGAATAGCAGATGT	50.0	36.4	58.3	56.5	450-457 bp	1.0	51.8
petD-rpoA_p5	TACAACAGCCCTTTGGTTGG	AGAGCATTTTCGAATAGCAGA	50.0	38.1	58.3	55.4	417-424 bp	1.0	51.7