Hotspot

rpl20-rps12

LSC · 55913-56730 · 818 bp

Back to Helixanthera

Pi 0.00504

MarkerSeek score 62.90

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 818
variable_sites: 4
pi: 0.00504
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TTTTGTTCTACGCCTCCGAG	CGCCGAGGAACATGTACTAG	50.0	55.0	57.9	57.9	873-893 bp	1.0	81.1
rpl20-rps12_p2	TCCTTGAATGAGCTCCTCGA	CGCCGAGGAACATGTACTAG	50.0	55.0	58.1	57.9	916-936 bp	1.0	80.4
rpl20-rps12_p3	GAGCTCCTCGAAAACTTGCT	CGCCGAGGAACATGTACTAG	50.0	55.0	58.2	57.9	907-927 bp	1.0	80.0
rpl20-rps12_p4	TCCTCGAAAACTTGCTGCAA	CGCCGAGGAACATGTACTAG	45.0	55.0	58.3	57.9	903-923 bp	1.0	79.0
rpl20-rps12_p5	TTTTGTTCTACGCCTCCGAG	GCCGAGGAACATGTACTAGG	50.0	55.0	57.9	57.5	872-892 bp	1.0	77.9