Hotspot

rps4-trnT-UGU

LSC · 38563-38973 · 411 bp

Back to Helixanthera

Pi 0.0101

MarkerSeek score 64.10

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 411
variable_sites: 4
pi: 0.0101
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	AGTTAATCCCGGTAAAGCGC	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.3	57.9	525-540 bp	1.0	79.1
rps4-trnT-UGU_p2	AGTTAATCCCGGTAAAGCGC	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.3	57.8	493-508 bp	1.0	78.4
rps4-trnT-UGU_p3	AGTTAATCCCGGTAAAGCGC	CCGCTTAGCTCAGAGGTTAG	50.0	55.0	58.3	57.5	533-548 bp	1.0	76.1
rps4-trnT-UGU_p4	AGTTAATCCCGGTAAAGCGC	CAGAGGTTAGAGCATCGCAT	50.0	50.0	58.3	57.5	523-538 bp	1.0	76.0
rps4-trnT-UGU_p5	AGTTAATCCCGGTAAAGCGC	TTAGGCGCGTATTATGTGGG	50.0	50.0	58.3	57.8	573 bp	0.5	56.0