Hotspot

psaA-ycf3

LSC · 34460-35087 · 628 bp

Back to Helixanthera

Pi 0.00489

MarkerSeek score 63.70

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 628
variable_sites: 3
pi: 0.00489
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	TGAGGCGCATAATTGGTTGA	50.0	45.0	57.9	57.9	747-751 bp	1.0	79.9
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	TTGAAGATCACGAGGCGTTT	50.0	45.0	57.9	57.8	731-735 bp	1.0	79.6
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	TTGGTTGAAGATCACGAGGC	50.0	50.0	57.9	58.2	735-739 bp	1.0	79.4
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	TGAAGATCACGAGGCGTTTC	50.0	50.0	57.9	58.3	730-734 bp	1.0	78.6
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	TCACGAGGCGTTTCGAATAA	50.0	45.0	57.9	57.6	724-728 bp	1.0	78.1