Hotspot

trnS-GCU-trnR-UCU

LSC · 4811-5163 · 353 bp

Back to Helixanthera

Pi 0.0472

MarkerSeek score 80.50

Primer available yes

Label trnS-GCU-trnR-UCU

Feature Metadata

label_name: trnS-GCU-trnR-UCU
feature_type: igs
region: LSC
length_bp: 353
variable_sites: 15
pi: 0.0472
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnR-UCU_p1	ATTAGCAATCCGCCGCTTTA	AGAAGACCTCTGTCCTATCCA	45.0	47.6	58.0	56.9	400-429 bp	1.0	70.2
trnS-GCU-trnR-UCU_p2	CGCATAACTCGTACAACGGA	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.1	56.9	419-448 bp	1.0	69.5
trnS-GCU-trnR-UCU_p3	ATTAGCAATCCGCCGCTTTA	AGGTTTAGAAGACCTCTGTCCT	45.0	45.5	58.0	57.9	406-435 bp	1.0	69.5
trnS-GCU-trnR-UCU_p4	CGCATAACTCGTACAACGGA	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.1	57.9	425-454 bp	1.0	68.8
trnS-GCU-trnR-UCU_p5	ACAAAACGGGAAAGAGAGGG	AGAAGACCTCTGTCCTATCCA	50.0	47.6	57.7	56.9	455-484 bp	1.0	68.0