Hotspot

trnL-UAG-ccsA

SSC · 114910-115023 · 114 bp

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Pi 0.0731

MarkerSeek score 82.00

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 114
variable_sites: 12
pi: 0.0731
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	TAGACACGCTGCTCTTAGGA	TTGAAACGATCGAAAAAGAAA	50.0	28.6	57.9	52.6	228-233 bp	1.0	45.9
trnL-UAG-ccsA_p2	GTAGACACGCTGCTCTTAGG	TTGAAACGATCGAAAAAGAAA	55.0	28.6	57.8	52.6	229-234 bp	1.0	45.9
trnL-UAG-ccsA_p3	AAATTGGTAGACACGCTGCT	TTGAAACGATCGAAAAAGAAA	45.0	28.6	57.8	52.6	235-240 bp	1.0	45.7
trnL-UAG-ccsA_p4	ACAAGCCGCTATGGTGAAAT	TTGAAACGATCGAAAAAGAAA	45.0	28.6	57.9	52.6	251-256 bp	1.0	45.3
trnL-UAG-ccsA_p5	GAGTTGAAGACAAGCCGCTA	TTGAAACGATCGAAAAAGAAA	50.0	28.6	57.9	52.6	260-265 bp	1.0	45.2