Hotspot

ndhF-rpl32

SSC · 113158-113950 · 793 bp

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Pi 0.0368
MarkerSeek score 72.80
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
793
variable_sites
42
pi
0.0368
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGCAAATAATAGAACGGCGC CGCTAAGGATTGGGTTGTCA 50.0 50.0 58.0 57.9 2162 bp 0.333333 52.2
ndhF-rpl32_p2 GGCAAATAATAGAACGGCGC TAAGGATTGGGTTGTCAGCG 50.0 50.0 58.0 57.9 2159 bp 0.333333 52.2
ndhF-rpl32_p3 CGGCGCATAAAGTAGCAAAG CGCTAAGGATTGGGTTGTCA 50.0 50.0 57.9 57.9 2148 bp 0.333333 51.7
ndhF-rpl32_p4 CGGCGCATAAAGTAGCAAAG TAAGGATTGGGTTGTCAGCG 50.0 50.0 57.9 57.9 2145 bp 0.333333 51.7
ndhF-rpl32_p5 GGCAAATAATAGAACGGCGC CATGCAGAATGTCCTCGCTA 50.0 50.0 58.0 57.8 2177 bp 0.333333 51.2