Hotspot

rpl16-rps3

LSC · 83545-83666 · 122 bp

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Pi 0.0519

MarkerSeek score 74.90

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 122
variable_sites: 9
pi: 0.0519
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	CTTTTGCATTCACGGGTTCC	CAAGTACAAATTGCAGGGCG	50.0	50.0	57.9	58.0	836-844 bp	1.0	83.1
rpl16-rps3_p2	AGGGTTAGCTCGTGACTTCT	CAAGTACAAATTGCAGGGCG	50.0	50.0	58.1	58.0	933-941 bp	1.0	83.0
rpl16-rps3_p3	CTTTTGCATTCACGGGTTCC	GTCGAGGGAATTGCACGTAT	50.0	50.0	57.9	58.1	1148-1156 bp	1.0	82.1
rpl16-rps3_p4	CCTTTTGCATTCACGGGTTC	CAAGTACAAATTGCAGGGCG	50.0	50.0	57.9	58.0	845 bp	0.333333	52.7
rpl16-rps3_p5	CCTTTTGCATTCACGGGTTC	GTCGAGGGAATTGCACGTAT	50.0	50.0	57.9	58.1	1157 bp	0.333333	52.3