Hotspot

trnS-GCU-trnG-GCC

LSC · 8031-8775 · 745 bp

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Pi 0.0385

MarkerSeek score 73.40

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 745
variable_sites: 41
pi: 0.0385
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	ATTAGCAATCCGCCGCTTTA	ACGAATCACACTTTTACCACT	45.0	38.1	58.0	55.5	819-887 bp	1.0	56.6
trnS-GCU-trnG-GCC_p2	CTTTAGTCCACTCAGCCACC	ACGAATCACACTTTTACCACT	55.0	38.1	57.9	55.5	804-872 bp	1.0	56.2
trnS-GCU-trnG-GCC_p3	ACGGAAAGAGAGGGATTCGA	ACGAATCACACTTTTACCACT	50.0	38.1	58.2	55.5	868-936 bp	1.0	55.4
trnS-GCU-trnG-GCC_p4	CCATCAACGGAAAGAGAGGG	ACGAATCACACTTTTACCACT	55.0	38.1	58.0	55.5	874 bp	0.333333	24.8
trnS-GCU-trnG-GCC_p5	CCATCAACGGAAAGAGAGGG	AACGAATCACACTTTTACCACT	55.0	36.4	58.0	56.2	875 bp	0.333333	22.9