Hotspot

ycf1

SSC · 122682-127847 · 5166 bp

Back to Heliophila

Pi 0.0439

MarkerSeek score 75.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5166
variable_sites: 873
pi: 0.0439
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTTGCCAGAACCAAACGGTA	GGATCTCTCGCGGAAGAATC	45.0	55.0	57.9	57.9	1184-1322 bp	0.6875	78.6
ycf1_p2	GTTTCGGATAGTGGAACCCC	GGATCTCTCGCGGAAGAATC	55.0	55.0	58.0	57.9	982-1120 bp	0.625	76.3
ycf1_p3	CCAGAACCAAACGGTAGGTC	GGATCTCTCGCGGAAGAATC	55.0	55.0	58.2	57.9	1225-1318 bp	0.4375	67.5
ycf1_p4	TGCCAAGGTTTCAGACGAAA	GGATCTCTCGCGGAAGAATC	45.0	55.0	58.0	57.9	1096-1111 bp	0.125	48.5
ycf1_p5	ATGCCAAGGTTTCAGACGAA	GGATCTCTCGCGGAAGAATC	45.0	55.0	57.7	57.9	1097-1112 bp	0.125	46.7