Hotspot

rpl32-trnL

SSC · 112241-113005 · 765 bp

Back to Heliophila

Pi 0.0658

MarkerSeek score 85.30

Primer available yes

Label rpl32-trnL

Feature Metadata

label_name: rpl32-trnL
feature_type: igs
region: SSC
length_bp: 765
variable_sites: 185
pi: 0.0658
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 85.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL_p1	GGAAGGGATATTGGACATCGT	TAACCACTGGGACTGGAGAG	47.6	55.0	57.2	58.1	1324-1650 bp	0.9375	74.8
rpl32-trnL_p2	GGAAGGGATATTGGACATCGT	AGATCTTTTGCCGCTACTCG	47.6	50.0	57.2	58.1	801-1127 bp	0.9375	74.8
rpl32-trnL_p3	GGAAGGGATATTGGACATCGT	TCCTAAGAGCAGCGTGTCTA	47.6	50.0	57.2	57.9	752-1078 bp	0.9375	74.3
rpl32-trnL_p4	GGAAGGGATATTGGACATCGT	AGCAGCGTGTCTACCAATTT	47.6	45.0	57.2	57.8	745-1071 bp	0.9375	73.8
rpl32-trnL_p5	GGAAGGGATATTGGACATCGT	CTAAGAGCAGCGTGTCTACC	47.6	55.0	57.2	57.8	750-1076 bp	0.9375	73.8