Hotspot

trnT-trnL

LSC · 45289-46117 · 829 bp

Back to Heliophila

Pi 0.0277

MarkerSeek score 80.70

Primer available yes

Label trnT-trnL

Feature Metadata

label_name: trnT-trnL
feature_type: igs
region: LSC
length_bp: 829
variable_sites: 109
pi: 0.0277
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-trnL_p1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCAATTCC	55.0	55.0	57.9	58.2	858-963 bp	1.0	89.7
trnT-trnL_p2	TATGGAAAAGCCGGCTATCG	CCGTAGCGTCTACCAATTCC	50.0	55.0	57.8	58.2	909-1014 bp	1.0	89.5
trnT-trnL_p3	GCGATGCTCTAACCTCTGAG	AGTCCGTAGCGTCTACCAAT	55.0	50.0	57.9	58.2	861-966 bp	1.0	89.0
trnT-trnL_p4	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCAATTCC	50.0	55.0	57.8	58.2	890-995 bp	1.0	88.9
trnT-trnL_p5	TATGGAAAAGCCGGCTATCG	AGTCCGTAGCGTCTACCAAT	50.0	50.0	57.8	58.2	912-1017 bp	1.0	88.8