Hotspot

rps4-trnT

LSC · 44746-45215 · 470 bp

Back to Heliophila

Pi 0.0417

MarkerSeek score 83.40

Primer available yes

Label rps4-trnT

Feature Metadata

label_name: rps4-trnT
feature_type: igs
region: LSC
length_bp: 470
variable_sites: 57
pi: 0.0417
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT_p1	TAAGATCGCTTCCTGCCCTA	CTCAGAGGTTAGAGCATCGC	50.0	55.0	57.9	57.9	499-630 bp	1.0	90.3
rps4-trnT_p2	TAAGATCGCTTCCTGCCCTA	ATGGTCATCGGTTCGATTCC	50.0	50.0	57.9	57.8	467-598 bp	1.0	89.4
rps4-trnT_p3	CGGAGCGTGATTGGTTTCTA	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.0	57.9	517-648 bp	0.9375	88.4
rps4-trnT_p4	CGGAGCGTGATTGGTTTCTA	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.0	57.8	485-616 bp	0.9375	87.5
rps4-trnT_p5	CGGAGCGTGATTGGTTTCTA	CCGCTTAGCTCAGAGGTTAG	50.0	55.0	58.0	57.5	525-656 bp	0.9375	85.5