Hotspot

trnI-CAU-rpl23

IRa · 160937-161561 · 625 bp

Back to Helicteres

Pi 0.0117
MarkerSeek score 66.50
Primer available yes
Label trnI-CAU-rpl23

Feature Metadata

label_name
trnI-CAU-rpl23
feature_type
igs
region
IRa
length_bp
625
variable_sites
6
pi
0.0117
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.82
markerseek_score
66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnI-CAU-rpl23_p1 GAATGGTTAAAGCGCCCAAC CCTAGTTGATCCTGATTCGACA 50.0 45.5 58.0 57.3 783-802 bp 1.0 63.3
trnI-CAU-rpl23_p2 GAATGGTTAAAGCGCCCAAC TGTCCTAGTTGATCCTGATTCG 50.0 45.5 58.0 57.3 786-805 bp 1.0 63.3
trnI-CAU-rpl23_p3 GAATGGTTAAAGCGCCCAAC CGACCCAATGCTTTATTTCTGT 50.0 40.9 58.0 57.3 805-824 bp 1.0 62.9
trnI-CAU-rpl23_p4 GAATGGTTAAAGCGCCCAAC GTCCTAGTTGATCCTGATTCGA 50.0 45.5 58.0 57.1 785-804 bp 1.0 61.2
trnI-CAU-rpl23_p5 GAATGGTTAAAGCGCCCAAC TCCTAGTTGATCCTGATTCGAC 50.0 45.5 58.0 57.1 784-803 bp 1.0 61.2