Hotspot

trnN-GUU-ndhF

IRb · 116861-117587 · 727 bp

Back to Helicteres

Pi 0.0259

MarkerSeek score 77.60

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: IRb
length_bp: 727
variable_sites: 18
pi: 0.0259
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	ACGACCAGTCAGTTAACAGC	GGGAAGCCGCATCTCTTTTT	50.0	50.0	57.8	58.8	846-946 bp	1.0	76.6
trnN-GUU-ndhF_p2	AGGATTCGAACCTACGACCA	GGGAAGCCGCATCTCTTTTT	50.0	50.0	58.2	58.8	859-959 bp	1.0	76.5
trnN-GUU-ndhF_p3	GGATTCGAACCTACGACCAG	GGGAAGCCGCATCTCTTTTT	55.0	50.0	57.8	58.8	858-958 bp	1.0	76.1
trnN-GUU-ndhF_p4	ACGACCAGTCAGTTAACAGC	AAATATGTAGGGGGAAGCCG	50.0	50.0	57.8	57.0	857-957 bp	1.0	75.5
trnN-GUU-ndhF_p5	AGGATTCGAACCTACGACCA	AAATATGTAGGGGGAAGCCG	50.0	50.0	58.2	57.0	870-970 bp	1.0	75.4