Hotspot

rpl23-trnI-CAU

IRb · 93067-93691 · 625 bp

Back to Helicteres

Pi 0.0714
MarkerSeek score 83.40
Primer available yes
Label rpl23-trnI-CAU

Feature Metadata

label_name
rpl23-trnI-CAU
feature_type
igs
region
IRb
length_bp
625
variable_sites
29
pi
0.0714
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.65
markerseek_score
83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl23-trnI-CAU_p1 CCTAGTTGATCCTGATTCGACA GAATGGTTAAAGCGCCCAAC 45.5 50.0 57.3 58.0 567-783 bp 1.0 66.1
rpl23-trnI-CAU_p2 TGTCCTAGTTGATCCTGATTCG GAATGGTTAAAGCGCCCAAC 45.5 50.0 57.3 58.0 570-786 bp 1.0 66.1
rpl23-trnI-CAU_p3 CGACCCAATGCTTTATTTCTGT GAATGGTTAAAGCGCCCAAC 40.9 50.0 57.3 58.0 589-805 bp 1.0 65.6
rpl23-trnI-CAU_p4 GTCCTAGTTGATCCTGATTCGA GAATGGTTAAAGCGCCCAAC 45.5 50.0 57.1 58.0 569-785 bp 1.0 64.0
rpl23-trnI-CAU_p5 TCCTAGTTGATCCTGATTCGAC GAATGGTTAAAGCGCCCAAC 45.5 50.0 57.1 58.0 568-784 bp 1.0 64.0