Hotspot

rpl22-rps19

LSC · 90875-90950 · 76 bp

Back to Helicteres

Pi 0.0263

MarkerSeek score 67.60

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 76
variable_sites: 2
pi: 0.0263
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	AGTTGGATATGGGTTCCTCCT	ACGGATCGTATGGTAGGACA	47.6	50.0	57.8	57.6	211 bp	1.0	68.7
rpl22-rps19_p2	AGTTGGATATGGGTTCCTCCT	TTGGGAGAATTCGCACCTAC	47.6	50.0	57.8	57.6	187 bp	1.0	68.5
rpl22-rps19_p3	AGTTGGATATGGGTTCCTCCT	TGGGAGAATTCGCACCTACT	47.6	50.0	57.8	58.4	186 bp	1.0	68.2
rpl22-rps19_p4	AGTTGGATATGGGTTCCTCCT	AATTGGGAGAATTCGCACCT	47.6	45.0	57.8	57.5	189 bp	1.0	67.5
rpl22-rps19_p5	GTTGGATATGGGTTCCTCCT	ACGGATCGTATGGTAGGACA	50.0	50.0	56.3	57.6	210 bp	1.0	64.1