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rps8-rpl14

LSC · 87342-87516 · 175 bp

Back to Helicteres

Pi 0.0514

MarkerSeek score 81.40

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 175
variable_sites: 9
pi: 0.0514
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	ATGCGAACCGTTCCTTTTCT	GCTCGGGAATTGAGACAGTT	45.0	50.0	58.1	57.9	315-322 bp	1.0	80.6
rps8-rpl14_p2	ATGCGAACCGTTCCTTTTCT	AATTTTTGGTGCGATCGCTC	45.0	45.0	58.1	57.7	331-338 bp	1.0	79.2
rps8-rpl14_p3	ATGCGAACCGTTCCTTTTCT	GAATTTTTGGTGCGATCGCT	45.0	45.0	58.1	57.7	332-339 bp	1.0	79.2
rps8-rpl14_p4	ATGCGAACCGTTCCTTTTCT	GATCGCTCGGGAATTGAGAC	45.0	55.0	58.1	58.5	319-326 bp	1.0	77.3
rps8-rpl14_p5	ATGCGAACCGTTCCTTTTCT	TCGAATTTTTGGTGCGATCG	45.0	45.0	58.1	57.5	334-341 bp	1.0	77.1