Hotspot

petA-psbJ

LSC · 70233-71377 · 1145 bp

Back to Helicteres

Pi 0.0122

MarkerSeek score 65.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1145
variable_sites: 14
pi: 0.0122
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CTACGTGTCCAAGGCCTTTT	ATTCTGGATTGGGTTCGTCC	50.0	50.0	58.1	57.6	1285-1294 bp	1.0	76.6
petA-psbJ_p2	GGCCTTTTGTTCTTCTTGGC	ATTCTGGATTGGGTTCGTCC	50.0	50.0	57.8	57.6	1273-1282 bp	1.0	76.3
petA-psbJ_p3	GATCCACTACGTGTCCAAGG	ATTCTGGATTGGGTTCGTCC	55.0	50.0	57.7	57.6	1291-1300 bp	1.0	75.1
petA-psbJ_p4	TACGTGTCCAAGGCCTTTTG	ATTCTGGATTGGGTTCGTCC	50.0	50.0	58.4	57.6	1284-1293 bp	1.0	74.3
petA-psbJ_p5	TGTCCAAGGCCTTTTGTTCT	ATTCTGGATTGGGTTCGTCC	45.0	50.0	57.5	57.6	1280-1289 bp	1.0	73.9