Hotspot

rbcL-accD

LSC · 62862-63683 · 822 bp

Back to Helicteres

Pi 0.0258

MarkerSeek score 59.60

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 822
variable_sites: 21
pi: 0.0258
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	TTGAATGCTACTGCGGGTAC	AGCCGGATCTTCGTTTACAC	50.0	50.0	58.0	58.0	1674-1723 bp	1.0	81.4
rbcL-accD_p2	TTGAATGCTACTGCGGGTAC	GCCGGATCTTCGTTTACACT	50.0	50.0	58.0	58.0	1673-1722 bp	1.0	81.4
rbcL-accD_p3	TTGAATGCTACTGCGGGTAC	TAGAGCTGTCACTCCAACCA	50.0	50.0	58.0	58.0	1720-1769 bp	1.0	81.3
rbcL-accD_p4	TTGAATGCTACTGCGGGTAC	ACCATTTCCACCTTCACCAC	50.0	50.0	58.0	58.0	2106 bp	0.5	59.8
rbcL-accD_p5	TTGAATGCTACTGCGGGTAC	CCATTTCCACCTTCACCACA	50.0	50.0	58.0	58.0	2105 bp	0.5	59.8