Hotspot

ndhC-trnV-UAC

LSC · 55958-57318 · 1361 bp

Back to Helicteres

Pi 0.012
MarkerSeek score 67.70
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1361
variable_sites
16
pi
0.012
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GGACCCTTTCTAATCGGAGC TGGGTCTTTGAAATGGGTCG 55.0 50.0 57.8 58.1 1560-1565 bp 1.0 79.6
ndhC-trnV-UAC_p2 GGACCCTTTCTAATCGGAGC GGGTCTTTGAAATGGGTCGA 55.0 50.0 57.8 57.8 1559-1564 bp 1.0 78.8
ndhC-trnV-UAC_p3 GGACCCTTTCTAATCGGAGC AATGCGTGTTGGGTCTTTGA 55.0 45.0 57.8 58.3 1569-1574 bp 1.0 77.8
ndhC-trnV-UAC_p4 GGACCCTTTCTAATCGGAGC ATGCGTGTTGGGTCTTTGAA 55.0 45.0 57.8 58.3 1568-1573 bp 1.0 77.8
ndhC-trnV-UAC_p5 GGACCCTTTCTAATCGGAGC TCTGTTTTACCGAGAAGGCC 55.0 50.0 57.8 57.5 1514-1519 bp 1.0 76.7