Hotspot

trnR-UCU-atpA

LSC · 10977-11255 · 279 bp

Back to Helicteres

Pi 0.0456
MarkerSeek score 70.70
Primer available yes
Label trnR-UCU-atpA

Feature Metadata

label_name
trnR-UCU-atpA
feature_type
igs
region
LSC
length_bp
279
variable_sites
12
pi
0.0456
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-atpA_p1 GGTTCAAATCCTATTGGACGC GTTTTCGACTTCAGGAACAA 47.6 40.0 57.3 54.1 308-324 bp 1.0 47.0
trnR-UCU-atpA_p2 GGTTCAAATCCTATTGGACGC GTTTTCGACTTCAGGAACAAT 47.6 38.1 57.3 54.7 308-324 bp 1.0 47.0
trnR-UCU-atpA_p3 GTTCAAATCCTATTGGACGCA GTTTTCGACTTCAGGAACAA 42.9 40.0 56.6 54.1 307-323 bp 1.0 47.0
trnR-UCU-atpA_p4 TGGATAGGACAGAGGTCTTCT GTTTTCGACTTCAGGAACAA 47.6 40.0 56.9 54.1 343-359 bp 1.0 46.4
trnR-UCU-atpA_p5 ATGAAAAGCGTCCATTGTCT GTTTTCGACTTCAGGAACAA 40.0 40.0 55.6 54.1 365-381 bp 1.0 46.0