Hotspot

rps16-trnQ-UUG

LSC · 6760-7452 · 693 bp

Back to Helicteres

Pi 0.00752
MarkerSeek score 66.90
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
693
variable_sites
5
pi
0.00752
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AGTGGTGGGTTTTGACATCC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 784-805 bp 1.0 82.7
rps16-trnQ-UUG_p2 TGCTTTCTACCACAGCGTTT GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.0 58.1 734-755 bp 1.0 82.6
rps16-trnQ-UUG_p3 TTGCTTTCTACCACAGCGTT GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.0 58.1 735-756 bp 1.0 82.6
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 745-766 bp 1.0 81.8
rps16-trnQ-UUG_p5 TTTTGACATCCACAGCCGAT GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.8 58.1 775-796 bp 1.0 81.2